|
Faster Separations:
Resolution vs. Column Length
5cm.
10cm.
Sample: uracil, acetophenone, methyl
benzoate, toluene, naphthalene
15cm.
25cm.
Sensitivity/Solvent
Consumption: 3mmID vs. 4.6mmID
|
System:
|
Beckman Altex 110 Pump
|
| |
Linear UV-106 Detector |
| |
Tubing: 0.007"ID
Inlet - 0.005"ID Outlet |
| |
Detection:
UV @ 254 |
| |
Setting:
0.2 AUFS |
| Back Pressure: |
about
1200psi for both columns |
| Mobile
Phase: |
60% Acetonitrile
40% Water
|
| Sample
Volume: |
5uL
|
| Sample: |
Uracil Phenol "N,N-Dimethylaniline"
Naphthalene |
| Material: |
Inertsil 5um ODS2 |
4.6mmID
3mmID
Different Variables For Each Chromatogram
| Flow Rate: |
1.0mL/min.(4.6mmID) |
|
0.5mL/min.(3mmID) |
|
Linear Velocity As Follows: |
|
6.02cm/min. (4.6mmID) |
|
7.07cm/min. (3mmID) |
| Sensitivity: |
See below; setting @ 0.2 AUFS
|
| Solvent Usage: |
60mL./hr. (4.6mmID) 30mL/hr.
(3mmID) |
| Column Dimensions: |
4.6mmID (150x4.6mmID) 3mmID
(150x3mmID) |
| |
|
| |
|
|
